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[FP-TH-08] Cataract - Miscellaneous and Investigative
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Apr 03 (Thu)
15:30 - 17:00
Room 12 - Tokyo International Forum 5F G510
Chair)David Allen


Duration 5min, Q&A 3min

Long Term Culturing of Both Yellow Tinted Blue Light Filtering and Clear Intraocular Lenses Using the Double-Faced Perfusion System

Rijo Hayashi
Rijo Hayashi Shimmin Hayashi Kiyomi Arai Shinichirou Yoshida Makoto Chikuda

Blue light filtering intraocular lenses are considered to protect retina from blue light damage after cataract surgery. The implantation of yellow tinted blue light filtering intraocular lenses (yellow IOLs) has accordingly become a common approach to cataract surgery. The biostability of yellow IOLs are therefore important. This is the first investigation comparing yellow IOLs and clear ultraviolet light filtering intraocular lenses (clear IOLs) after long term culturing using a double-faced perfusion system.

Six hydrophobic acrylic IOLs were included. Three clear IOLs, an AF-1 VA-60BB (HOYA), an AcrySof® SA60AT (Alcon) and a Nex-Acri™ N4-18B (NIDEK), in addition, three yellow IOLs, an AF-1 YA-60BB (HOYA), an AcrySof® SN60AT (Alcon) and a Nex-Acri™ AA N4-11YB (NIDEK). Of the three clear and three yellow IOLs used, each yellow IOL had a matching counterpart clear IOL of the same stock, for example, a yellow AF-1 YA-60BB IOL and a clear AF-1 VA-60BB.The only difference being the lens color.

The IOLs were cultured under 37℃ for 7 months using a double-faced perfusion culture system. This culture system was originally designed for culturing lenses by immersing the anterior surface of the lens in a culture base which imitates the aqueous humor and immersing the posterior surface of lens in a culture base which imitates the vitreous. The IOLs are kept inside lens capsule and do not come into contact with the vitreous in vivo, both the anterior and posterior surfaces of IOLs cultured in this study were immersed in our artificial aqueous humor. The artificial aqueous humor was perfused at the rate of 2μl per minute which is the same rate that human aqueous humor is exchanged. This system cultured the IOLs in conditions which replicate the intraocular environment. Resolution, light transmittance rate and lens power in air were measured before and after culturing. Surface coarseness of the anterior and posterior surfaces was also measured.

Results and Conclusion
There were no differences of resolution, lens power in air or surface coarseness between clear and yellow IOLs, neither before nor after culturing. After culturing for 7 months, there were no changes in the resolution, light transmittance rate and lens power in air. The surface coarseness of the anterior and posterior surface increased after culturing, however, it was not clinically significant.

[ Keyword ]
yellow tinted IOL / organ culture

[ Conflict of Interest ]

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