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[FP-SA-43] Ocular Imaging
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Apr 05 (Sat)
15:30 - 17:00
Room 13 - Tokyo International Forum 4F G402
Ocular Imaging
Chair)Yoko Miura、Chair)Dirk-Uwe Bartsch


Duration 5min, Q&A 3min

Fluorescence Lifetime Imaging in Eyes After Successful Macula-Off Retinal Detachment Repair and in Unaffected Partner Eyes

Marcel Menke
Marcel Menke Chantal Dysli Martin Zinkernagel Jens Kowal Sebastian Wolf

The fluorescence of organic molecules is not only characterized by the emission spectrum, it has also a specific decay rate or lifetime. Fluorescence lifetime imaging ophthalmoscopy (FLIO) is a novel imaging method that allows measurements of fluorescence lifetimes in vivo in the retina. The instrument used for FLIO imaging is based on a Heidelberg Engineering Spectralis® system. In this study, we assessed fluorescence lifetimes in eyes after successful macula-off retinal detachment surgery. Values were compared to the unaffected partner eyes.

Twenty eyes of 10 patients after successful macula-off retinal detachment repair have been included 6-8 weeks after surgery. Unaffected partner eyes were used as controls.

Fluorescence decay times were measured in a short wavelength channel (498 - 560 nm) and in a long wavelength channel (560 - 720 nm). Short fluorescent lifetimes (tau1), long fluorescence lifetimes (tau2), as well as corresponding amplitudes were calculated for each acquired pixel within the retina by time-correlated single photon counting. For comparison purposes an ETDRS grid was used to average all values for 3 zones (center, inner ring, outer ring). Values of affected eyes were compared with corresponding healthy retina of partner eyes using a student's t test.

Results and Conclusion
Mean tau1 lifetimes (in picoseconds) in the short wavelength channel were significantly longer in all ETDRS zones of affected eyes (center: 231.7 ± 17.64; inner ring: 288.0 ± 19.67; outer ring: 301.4 ± 15.88) compared to controls (center: 182.6 ± 13.63, p<0.041; inner ring: 240.4 ± 10.51, p<0.047; outer ring: 262.3 ± 9.420, p<0.049). In the long wavelength channel, tau1 differences were also significant for the ETDRS center. (affected eyes: 278.2 ± 17.44; controls: 223.3 ± 14.49, p<0.026). Mean amplitudes have been found to be significantly higher in affected eyes compared to controls. Mean amplitudes in the center were 0.002485 ± 0.0003058 in affected eyes and 0.001614 ± 0.0002483 in controls (p<0.041). In the inner ETDRS ring mean amplitudes were 0.002496 ± 0.0002823 in affected eyes and 0.001606 ± 0.0002334 in controls (p<0.025). In the outer ETDRS ring mean amplitudes were 0.002740 ± 0.000284 in affected eyes and 0.001791 ± 0.0002641 in controls (p<0.025). All other values showed no statistically significant difference.
 In conclusion, we were able to show that macula-off retinal detachment leads to specific and quantifiable changes in fluorescence lifetimes in the macula, in particular in short fluorescence lifetimes (tau 1) and amplitudes.

[ Keyword ]
Fluorescence lifetime imaging / Macula / retinal detachment

[ Conflict of Interest ]

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