Presentation Information

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[FP-SA-42] New Technology
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Day
Apr 05 (Sat)
Time
13:30 - 15:00
Room
Room 13 - Tokyo International Forum 4F G402
Topic
New Technology and Alternative Medicine
Chair/Coordinator
Chair)Yuko Seko、Chair)Stephan Kaminski
 
 
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FP-SA-42-2

Duration 5min, Q&A 3min

Subretinal Implantation of Gelatin Films with Stem Cells Derived RPE in Rats

【Speaker】
Yuntao Hu
【Author】
Yuntao Hu Biju Thomas Dennis Clegg David Hinton Mark Humayun


Objective/Purpose
Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells were cultured on gelatin films and implanted into the subetinal space in rats in order to monitor post-implantation cells fate and gelatin degradation phenomenon.

Materials/Patients
Monolayer cultures of hESC-RPE cells were cultured on 27 μm thickness gelatin films.

Methods
RCS rats were randomly divided into two groups and subretinally implanted with gelatin films without the cells (Group A, n=9) or gelatin films with monolayer hESC-RPE cells (Group B, n=9). One week, one month and three months post-implantation OCT scanning and histological examinations were performed. cultures

Results and Conclusion
The gelatin without the cells did not degrade in subretinal space up to 3 months after implantation (Fig1, 2). Degradation of the gelatin with hESC-RPE cells was observed at 3 months after implantation (Fig3, 4). However, the hESC-RPE cells maintained monolayer RPE morphology and were RPE65 positive.
Degradation of subretinally implanted gelatin scaffolds is cell-dependent and may be related to the release of proteolytic enzymes or other factors by hESC-RPE cells.

[ Keyword ]
Stem cells / Retinal pigment epithelial cells / Degradation / Transplantation / Gelatin

[ Conflict of Interest ]
No

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