Duration 5min, Q&A 3min
Subretinal Implantation of Gelatin Films with Stem Cells Derived RPE in Rats
Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells were cultured on gelatin films and implanted into the subetinal space in rats in order to monitor post-implantation cells fate and gelatin degradation phenomenon.
Monolayer cultures of hESC-RPE cells were cultured on 27 μm thickness gelatin films.
RCS rats were randomly divided into two groups and subretinally implanted with gelatin films without the cells (Group A, n=9) or gelatin films with monolayer hESC-RPE cells (Group B, n=9). One week, one month and three months post-implantation OCT scanning and histological examinations were performed. cultures
Results and Conclusion
The gelatin without the cells did not degrade in subretinal space up to 3 months after implantation (Fig1, 2). Degradation of the gelatin with hESC-RPE cells was observed at 3 months after implantation (Fig3, 4). However, the hESC-RPE cells maintained monolayer RPE morphology and were RPE65 positive.
Degradation of subretinally implanted gelatin scaffolds is cell-dependent and may be related to the release of proteolytic enzymes or other factors by hESC-RPE cells.
[ Keyword ]
Stem cells / Retinal pigment epithelial cells / Degradation / Transplantation / Gelatin
[ Conflict of Interest ]