Presentation Information

Free Paper

 

[FP-SA-35] Pharmacology
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Day
Apr 05 (Sat)
Time
15:30 - 17:00
Room
Room 2 - Tokyo International Forum 4,5F Hall C
Topic
Pharmacology, Ocular Drug Delivery
Chair/Coordinator
Chair)John Christoforidis
 
 
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FP-SA-35-10

Duration 5min, Q&A 3min

Curcumin Loaded MePEG-PCL Nanoparticle Prevents Posterior Capsular Opacification

【Speaker】
Aditya Konar
【Author】
Aditya Konar Sushovan Chowdhary Rajdeep Guha Sarbani Hazra


Objective/Purpose
Cytokines and growth factors released after cataract surgery have been identified as major contributors to the development of PCO. Curcumin has demonstrated its anti inflammatory effect in different cells. This study was designed to evaluate the anti inflammatory effect of curcumin on LPS induced lens epithelial cells (LECs) and further, to explore effect of curcumin nanoparticle for prevention of posterior capsular opacification in rabbits.

Materials/Patients
In vitro testing with free curcumin and curcumin nanoparticles was done in lens epithelial cells.In vivo study was conducted in New Zealand White rabbits, with prior permission of Institutional Animal Ethics Committee and as per guidelines of Association of research in Vision & Opthalmology (ARVO)

Methods
MePEG-PCL nanoparticle (NP) of curcumin was prepared and characterized. In-vitro uptake of free curcumin and curcumin NP by lens epithelial cells and retention of curcumin within the cells was examined. Expression of pro-inflammatory cytokines and modulation of NFkB signaling by LPS induced lens epithelial cells with or without pre-incubation with curcumin was assessed by ELISA, western blotting and immunocytochemistry. Curcumin nanoparticle (NP) was injected intracamerally following lensectomy in rabbit eye and PCO was evaluated by slit lamp biomicroscopy and histopathology. The toxicity of curcumin nanoparticle on the intraocular structures was evaluated clinically, by tonometry, specular microscopy, electroretinography and histopathology.

Results and Conclusion
The size and zeta potential of MePEG-PCL curcumin nanoparticles were 84.61± 0.79 nm, 39 ±3.12 respectively. Cellular uptake of free curcumin and curcumin nanoparticle was similar but enhanced retention was observed with curcumin nanoparticles. Curcumin prevented the increased expression of IL1β, IL6, TNFα and reduced phosphorylation of IkBα and nuclear localization of NFkB in LPS induced LECs. Treatment with curcumin nanoparticle showed reduction in the degree of PCO clinically and histology of the lens capsule showed decreased proliferation and migration of LECs. Mean IOP, corneal endothelial cell number and morphology and ERG of the curcumin NP treated eyes were not significantly different from baseline lines values. MePEG-PCL curcumin nanoparticle reduces PCO without causing any toxicity to intraocular structures

[ Keyword ]
Curcumin nanoparticles prevention of posterior capsular opacification

[ Conflict of Interest ]
No

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