演題番号 : P1-m14
野中 綾子 / Ayako Nonaka:1 山本 はるか / Haruka Yamamoto:1 松木 則夫 / Norio Matsuki:1 野村 洋 / Hiroshi Nomura:1
1:東京大院・薬・薬品作用学 / Lab of Chemical Pharmacology, Grad Sch of Pharmaceutical Sciences, The Univ of Tokyo, Tokyo
Although memory is presumably stored in the subset of neurons during memory formation and read out from the same subset during memory retrieval, there is no clear evidence to confirm the idea. To establish this hypothesis, we identified neuronal populations engaged in memory formation and retrieval detecting Arc RNA, which is a neural activity marker. Mice were conditioned with footshock in a conditioning chamber. They were returned to homecage and 25 min later re-exposed to the conditioning chamber for 5 min to recall the memory. Immediately after the retrieval, we removed the brains and detected Arc RNA in the lateral amygdala by fluorescence in situ hybridization. The method enables us to distinguish neurons activated in each process. The activity of individual neurons during conditioning and re-exposure were determined by subcellular localization of Arc RNA. A number of neurons activated during conditioning were also activated during re-exposure. This result suggests that a read-out of neuronal ensemble organized during memory formation may lead to memory retrieval.