Intracellular mechanisms underlying the inhibitory effect of alpha-2A-adrenoceptor stimulation on excitatory synaptic transmission in the rat medial prefrontal cortex
演題番号 : P1-b08
1:Institute of Neurobiology,Fudan University.
Stimulation of alpha-2A-adrenoceptors (ARs) in the prefrontal cortex (PFC) produces a beneficial effect on cognitive functions such as working memory. Alpha-2-adrenergic agonist guanfacine has been used experimentally and clinically for treatment of psychiatric disorders such as attention-deficit/hyperactivity disorder (ADHD). A previous study in our laboratory showed that stimulation of alpha-2A-adrenoceptors suppresses excitatory synaptic transmission in the medial prefrontal cortex (mPFC) of rat. However, the underlying mechanism is still unclear. In the present study, we recorded evoked excitatory postsynaptic current (eEPSC) in the mPFC of rats, using whole-cell patch-clamp recording technique. Our results show that stimulation of alpha-2A-AR inhibits excitatory synaptic transmission in the mPFC through cAMP-PKA-PP1-AMPA-R and cAMP-PKA-PP1-CAMKII-AMPA-R signaling pathways.