Expression of Class II & IV HDACs during murine brain development
演題番号 : P1-d17
Afsaneh Goudarzi:1 Farzam Ajamian:1
1:Dept Biology, Univ of Guilan, Rasht, Iran.
Histone deacetylases (HDACs) involved in the remodeling of nuclear chromatin and thus have a key role in the regulation of gene expression in all eukaryotic cells including neural. In central nervous system (CNS) this phenomenon therefore, reflects their key contribution in neural cell proliferation and/or differentiation in important processes such as fetal brain formation and development. Histone hypoacetylation seems to be important to oligodendrocyte lineage development and a correlation between reduction in acetylation of histone core proteins and development of neural cells in CNS was recently addressed. Using reverse transcription-polymerase chain reaction (RT-PCR) we investigated the expression of all known member of Class II HDACs during early differentiation of embryonic murine neural progenitors in Vitro. Our initial screen showed that the members of this family are highly expressed during this time window, while by far the highest expression level was belonging to the HDAC9. We are currently working to measure the expression levels of these members from tissues in vivo in distinct part of murine brain including cortex during pre- and post-natal development. The Zn-dependent HDAC 11 in mammals is the only member of class IV HDACs. In developing murine brain, HDAC11 is shown to be widely expressed postnatal and its expression correlates with the maturation of neural cells. However, little is known about HDAC11 role in prenatal development of muring brain. We are currently investigating the change of HDAC11 expression levels during three main time points of mouse brain development; days 14, 18 and 21 in vivo, in cortex tissues. Our data together with others suggest that the member of class II and IV HDACs may act as important regulators of gene expression in developing neural cells and tissues in murine CNS.