演題番号 : P1-d11
稲川 優多 / Yuta Inagawa:1 長井 薫 / Kaoru Nagai:1
1:山梨大学医学工学総合研究部 環境遺伝医学講座 / Department of Epigenetic Medicine, Interdisciplinary Graduate School of Medicine and Engineering, The University of Yamanashi
Epigenetic regulation, such as histone modification and DNA methylation, is essential in the central nervous system (CNS). Although importance of epigenetics on neuronal or astroglial differentiation from neural precursor cells has been reported, contribution of epigenetics on the differentiation from oligodendrocyte precursor cells (OPCs) has not been studied, yet. In this study, we investigated the effect of valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, on the differentiation of OPCs model cell line CG4-16 cells. VPA reduced cell number dose dependently. And the reduction was inhibited by DNA polymerase inhibitor aphidicolin. VPA increased dendritic number, and upregulated oligodendrocyte marker, MAG on oligodendrocyte differentiating condition. While, on OPCs maintaining condition, VPA altered the cell shape to epithelial like morphology, and upregulated an astrocyte marker, GFAP. Then, we evaluated if the effect of VPA was dependent on HDAC activity. Valpromide, which is a VPA derivative without HDAC inhibitory activity, did not show any effect on CG4-16 cells. Our data suggests that VPA arrested cell growth and promoted glial differentiation from OPCs via its inhibitory activity of histone deacetylation.