Intravenous Neural Stem Cell Transfusion Restores Functional Defecits in 6-OHDA Lesioned Rats
演題番号 : P1-d09
Kavita Seth:1 Anshi Shukla:1 Reyaz_W Ansari:1 Rajnish_K Chaturvedi:1 Ashok_K Agrawal:1
We explored the possibility of migration of intravenously infused NSCs to lesioned site in 6-OHDA lesioned rat model of PD in order to restore functional deficits as it would be less traumatic and more useful in widely spread or multifocally present lesions.In-vitro expended NSCs, labeled with PKH2 linker dye were intravenously infused through tail vein into 6-OHDA-lesioned rats. NSCs could be tracked in ipsilateral striatum indicating their migration to lesioned area. NSC infusion led to significant functional restoration as evident from as evident from significant decrease in D-amphetamine induced rotations along with recovered spontaneous locomotion and grip strength in infused animals. This was accompanied by significant up-regulation in the expression of tyrosine hydroxylase , its neurotrophin regulators such as GDNF / BDNF along with restored Bcl-2 and Bax expression as assessed through immunohistochemistry, western blot and RT-PCR. The present study show that intravenously infused NSC could migrate to lesioned site leading to functional restoration in rat model of PD. These findings can help in advancing stem cell concepts toward clinical relevance leading to the less traumatic and cost effective PD management.