演題詳細

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神経伝達物質・修飾物質
Neurotransmitters and Modulators

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開催日
2010年09月02日(木)
時 間
11:00 - 12:00
会 場
Poster Room 1

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神経細胞におけるトランスサイトーシス:生体内Amyloid-βへの応用
Neuronal transcytosis of protein: application for Amyloid-β in vivo

演題番号 : P1-b17

松本 由樹 / Yoshiki Matsumoto:1 三木 崇範 / Takanori Miki:1 割田 克彦 / Katsuhiko Warita:1 矢倉 富子 / Tomiko Yakura:1 劉 俊騫 / Jun-Qian Liu:1 竹内 義喜 / Yoshiki Takeuchi:1 

1:香川大・医・神経機能形態 / Dept Anatomy and Neurobiology, Kagawa Univ 

 

Neuronal transcytosis of protein was demonstrated using the WGA-HRP method. The transcytosis at synapses was observed in coincidence with the stage of no neurotransmitter release after injection of WGA-HRP into the vagus nerve (VN). Further experiments were performed after co-injection of Rab3A-siRNA with WGA-HRP into the VN. This injection was involved in detecting the transcytosis of WGA-HRP including the passing type crossing the synapses and secretion type followed by endocytosis of postsynaptic membranes. The present results raised a possibility that WGA plays an important role in transcytosis of protein. Therefore, considering WGA to be a valuable tool for therapeutic drug targeting via transcytosis, WGA conjugated Amyloid β (WGA-Aβ) was investigated to decrease amyloid deposits in Alzheimer's disease. It was confirmed whether 1) WGA conjugates amyloid-β, 2) WGA-Aβ moves to terminals by axonal flow as well as WGA-HRP and 3) WGA-Aβ shows transcytosis to secondary neurons. WGA-Aβ was observed in terminals in the nucleus of solitary tract (NST) after co-injection of fluorescence Aβ (FAβ) and WGA (FWGA) into the VN, or injection of FAβ into the VN and FWGA into the common carotid artery, respectively. It was of particular interest that WGA-Aβ seems to show transcytosis to neurons of the NST.

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