演題番号 : P1-b07
國枝 恒兵 / Kouhei Kunieda:1 井田 智章 / Tomoaki Ida:1 澤 智裕 / Tomohiro Sawa:2 赤池 孝章 / Takaaki Akaike:2 板倉 誠 / Makoto Itakura:3 高橋 正身 / Masami Takahashi:3 居原 秀 / Hideshi Ihara:1
1:大阪府大院・理・生物科学 / Dept. of Biol. Sci., Grad. Sch. of Sci., Osaka Pref. Univ., Osaka 2:熊本大院・医薬・微生物学 / Dept. of Microbiol., Grad. Sch. Med. Sci., Kumamoto Univ., Kumamoto 3:北里大・医・生化学 / Dept. of Biochem., Kitasato Univ. Sch. of Med., Kanagawa
[Background] Exocytosis of the neurotransmitter is triggered by the formation of a stable trimer complex of v-SNARE(synaptobrevin on synaptic vesicle) and t-SNAREs(SNAP25 and syntaxin on the presynaptic membrane). Formation of the SNARE complex is a crucial step in neurotransmitter release, and this process is regulated by several factors. We detected 8-nitro-cGMP, which is formed in an NO-dependent manner, as a novel second messenger in the cells. In addition to functioning as a cGMP analog, 8-nitro-cGMP plays an important role in membrane permeability and protein S-guanylation. We found that 8-nitro-cGMP regulates exocytosis. In this study, we examined the effect of 8-nitro-cGMP on the formation of the SNARE complex and the S-guanylation of SNARE proteins.