演題番号 : P3-d08
荒井 光徳 / Mitsunori D Arai:1 駒井 章治 / Shoji Komai:1
1:奈良先端大・バイオ・細胞構造学 / Graduate School of Biological Sciences, Division of Structural Cellular Biology, NAIST
Many researchers have studied about neural stem cells (NSCs) and its transplantation into several brain regions to treat neurological disorders, such as Parkinson's disease, ischemia, and spinal cord injuries, caused by damage or loss of neurons and glial cells. These therapies indeed have been helped to recovery of motor functions such as dyskinesia. The recovery have been shown by testing roterod or BBB scale and some behavioral tests. However, little is known about contribution of transplantation to recover motor functions and how neural activities form the appropriate local neural circuits with transplanted cells correctly processing the information with endogenous neurons. It is essential for not only genetic factor but also experience after born to develop correct neural circuits. Therefore we examined whether transplanted NSCs in the intact somatosensory cortex could be involved in functional neural circuits morphologically and physiologically manipulated by using of genetic factors and neural activities. Firstly we investigated whether Neurogenin2 and Mash-1 can differentiate transplanted NSCs either into excitatory or inhibitory neurons, which are thought to regulate differentiation of excitatory and inhibitory neuron respectively in cortical development. Moreover we examined how influence the differentiation of transplanted cells given sensory inputs in diversity ways. In this report we analyzed histologically what kind of cell types NSCs differentatied after gene manipulations and environmental enrichment. This approach will allow us to reveal the importance of the experience or neuronal activity for building up the appropriate neuronal circuit in the cortex. Additionally this method could be helpful to care the patients suffered from neurological disorders.