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神経幹・前駆細胞と細胞分化、移植III
Neural Stem/Progenitor Cells and Cellular Differentiation, Transplantation III

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開催日
2010年09月04日(土)
時 間
13:00 - 14:00
会 場
Poster Room 1

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マウス虚血脳へのiPS細胞移植
Induced pluripotent stem cells transplanted in mouse ischemic brain

演題番号 : P3-d06

河相 裕美 / Hiromi Kawai:1 山下 徹 / Toru Yamashita:1 太田 康之 / Yasuyuki Ohta:1 出口 健太郎 / Kentaro Deguchi:1 出口 章子 / Shoko Deguchi:1 張 雪梅 / Xuemei Zhang:1 池田 佳生 / Yoshio Ikeda:1 松浦 徹 / Tohru Matsuura:1 阿部 康二 / Koji Abe:1 

1:岡山大学大学院医歯薬学総合研究科 脳神経内科学 / Department of Neurology , Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University 

 

Stroke is a major neurologic disorder. Induced pluripotent stem (iPS) cells can be produced frombasically any part of patients, with high reproduction ability and pluripotency to differentiate into various types of cells, suggesting that iPS cells can provide a hopeful therapy for celltransplantation. However, transplantation of iPS cells into ischemic brain has not been reported. In this study, we showed that the iPS cells fate in a mouse model of transient middle cerebralartery occlusion (MCAO). Undifferentiated iPS cells (5×105) were transplanted into ipsilateral striatum and cortex at 24 h after 30 mins of transient MCAO. Behavioral and histologic analyses were performed at 28 day after the cell transplantation. To our surprise, the transplanted iPS cells expanded and formed much larger tumors in mice postischemic brain than in sham-operated brain. The clinical recovery of the MCAO+iPS group was delayed as compared with the MCAO+PBS(phosphate-buffered saline) group. iPS cells formed tridermal teratoma, but could supply a great number of Dcx-positive neuroblasts and a few mature neurons in the ischemic lesion. iPS cells have a promising potential to provide neural cells after ischemic brain injury, if tumorigenesis is properly controlled.

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