演題番号 : P3-d04
森田 晶子 / Shoko Morita:1 鵜飼 真璃 / Shinri Ukai:1 宮田 清司 / Seiji Miyata:1
1:京都工芸繊維大学 応用生物学部門 / Department of Applied Biology, Kyoto Institute of Technology, Kyoto
Neurogenesis in adult mammalian nervous system is well known in the subventricular zone of the anterolateral ventricle and subgranular zone of the hippocampus. The circumventricular organs (CVOs) are brain areas that lack the BBB or tight junctions between endothelial cells, and therefore are able to access to circulating substances in blood and cerebrospinal fluids. Recently, it is shown that adult CVOs cells are able to proliferate and undergo constitutive neurogenesis and gliogenesis. Nerve/glial antigen 2 (NG2) is highly expressed in immature and angiogenic microvascular pericytes at early developmental period of the central nervous system (CNS), but its expression becomes lower in microvascular pericytes of the adult CNS. In the present study, we found the prominent NG2 expression and determined the fate of bromodeoxyuridine positive cells in adult CVOs such as the subfornical organ (SFO), organum vasculosum of the lamina terminalis (OVLT), median eminence (ME), area postrema (AP), neurohypophysis (NH), and pineal gland (PG), using confocal microscopic immunohistochemistry. Furthermore, we analyzed the cell proliferation changes by salt-loading, known as the chronic stimulation to cause the structural remodeling. The present study thus demonstrates that NG2 is highly expressed at microvessels of the CVOs in adult CNS and suggests that microvessels of the CVOs may possess the immature feature to reconstruct microvascular structures. Cells may be able to undergo not only neurogenesis and gliogenesis, but also angiogenesis in adult CVOs.