演題番号 : P3-c28
鈴木 純子 / Junko Suzuki:1 石堂 正美 / Masami Ishido:1
1:国立環境研 / Natl Inst for Environmental Studies, Tsukuba, Japan
Developmental neurotoxicity of environmental chemicals has been recognized worldwide as a serious threat to human health. The large numbers of environmental chemicals that now exist have made assessing their neurodevelopmental risks challenging. Therefore, in the previous study we have established an in vitro neurosphere assay system to evaluate for environmental chemical toxicity. In this study, we extended to quantify neurodevelopmental toxicity of endocrine disruptors, such as bisphenol A. Neural stem cells isolated from rat E16 mesencephalone were plated in serum-free DMEM/ F12 (1:1) medium in the presence of bFGF and EGF to form neurospheres. Cells emerged from the neurosphere in adhesive dishes and migrated along the radial axis. The migrating populations were positive for nestin, a marker of neural stem cells. Exposure to bisphenol A resulted in inhibition of cell migration and proliferation in a dose-dependent manner. Quantitative analysis revealed a linear function between the inhibition of migration and the logarithm of bisphnol A concentration (0-100 µm); the percentage of inhibition by 1 µM bisphnol A of migration was 35% (p<0.05). Thus, we showed for the first time that bisphenol A inhibited migration as well as proliferation of neural stem cells in vitro and that a neurosphere assay system in vitro is very useful for rapid quantification of neurodevelopmental toxicity of environmental chemicals.