演題番号 : P3-c26
王 飛霏 / Feifei Wang:1 安原 隆雄 / Takao Yasuhara:1 亀田 雅博 / Masahiro Kameda:1 菊池 陽一郎 / Yoichiro Kikuchi:1 Judith Tayra:1 Hanbai Liang:1 新光 阿以子 / Aiko Shiko:1 三好 康之 / Yasuyuki Miyoshi:1 伊達 勲 / Isao Date:1
1:岡山大学大学院 脳神経外科 / Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Cell transplantation is known as an effective therapy for disorders of the central nervous system in animal studies. For improving the efficacy of cell transplantation, enhancement of cell survival after transplantation is important. In the present study, we investigated whether GDNF (glial cell-derived neurotrophic factor)-pretreatment with neural stem cells (NSCs) enhanced the survival of transplanted cells using Q-dot (Quantum dot) imaging in Parkinson's disease (PD) model of rats. At first, we examined neuroprotective effects of GDNF on oxygen-glucose deprivation (OGD) because cells were exposed by hypoxic-ischemic damage in the process of implantation. NSCs were pretreated with 10-1000 ng/ml of GDNF before OGD-induced hypoxic-ischemic damage. At 12 hours after OGD, NSCs pretreated with 10, 100 and 500 ng/ml of GDNF showed significant increments of survival rate, compared with NSCs pretreated with PBS. PD model of rats were established by unilateral injection of 6-hydroxydopamine (6-OHDA, 9μg) into the medial forebrain bundle. NSCs were labeled by 10 nM of Q-dot solution (Q tracker 525) after pretreatment with 100 ng/ml of GDNF for 3 days. At 2 weeks after 6-OHDA injection, GDNF-pretreated NSCs, NSCs andor PBS were injected into the striatum of PD model of rats. Q-dot-labeled NSCs could be observed brilliantly in the striatum at 1 week after transplantation. The survival of transplanted cells was significantly ameliorated in GDNF-pretreated NSCs group, compared with that of PBS-pretreated NSCs group. Many of Q-dot/Nestin, Q-dot/GFAP and Q-dot/Dcx double-positive cells were observed in both the GDNF-pretreated NSCs group and PBS-pretreated NSCs group. There was no significant difference in the cell differentiation between GDNF-pretreated NSCs group and PBS-pretreated NSCs group. These results demonstrated that GDNF-pretreatment ameliorated the survival of NSCs following transplantation and that Q-dot might provide a useful tool for imaging of transplanted cells.