Effects of periadolescent exposure to methamphetamine on adult behavioral performances in male Akt1 deficient mice
演題番号 : P1-p14
Wan-Ting Chang:1 Yi-Wen Chen:1 Ju-Chun Pei:1 Wen-Sung Lai:1,2
1:Psychology, National Taiwan University 2:Neurobiology and Cognitive Science Center, National Taiwan University, Taipei, Taiwan
Schizophrenia appears to be a multifactorial disorder with a strong genetic predisposition. Accumulating evidence suggests that Akt1 might contribute to susceptibility for schizophrenia. While evidence indicates a genetic basis for schizophrenia, the concordance rate in homozygotic twins inflicting schizophrenia never reaches 50%, suggesting epigenetic involvement in this disorder, especially during early development and before adolescence. Periadolescent period is crucial for the maturation of neural system which appears to be more vulnerable to drug abuse and environmental insults. The objective of this study is to use periadolescent exposure to methamphetamine (MA, a dopamine agonist) as an environmental insult to investigate gene-environment interaction in Akt1 deficient mice. Akt1 heterozygous (HET) mice and wild type (WT) littermate controls were generated from Akt1 HET breeding pairs. During periadolescent period (postnatal day 35~45), each subject received 6 injections of either saline or MA (2 mg/kg, s.c.) daily to induce behavioral sensitization, and their behavioral performances were examined during adolescence and after adulthood. A comprehensive battery of behavioral tasks was conducted to examine their behavioral phenotypes. Our preliminary data revealed that both HET and WT mice exhibited behavioral sensitization to MA during periadolescence, and HET mice with MA exposure appeared to be less anxious in the open field with 1 mg/kg MA challenge. In adulthood, the 4 groups appear to have normal basic behavioral phenotypes, except HET mice with periadolescent exposure to MA displayed less anxious behavior in the elevated plus maze. Further experiments are in progress to further elucidate gene-environment interaction in these mice.