Behavioral and cognitive characterizations of AKT1-NRG1 single and double mutant mice
演題番号 : P1-p04
Ching-Hsun Huang:1 Yi-Wen Chen:1 Ju-Chun Pei:1 Wen-Sung Lai:1,2
1:Department of Psychology, National Taiwan University, Taipei, Taiwan 2:Neurobiology and Cognitive Science Center, National Taiwan University, Taipei, Taiwan
Accumulating evidence from human genetics and animal studies suggest that both AKT1 and NRG1 (neuregulin 1) might contribute to susceptibility for schizophrenia. Recent findings indicated that NRG1 acts through ErbB2/4 in a paracrine fashion to stimulate PI3-kinase/AKT signaling pathway, which might be involved in the pathogenesis of schizophrenia. However, the biological functions and the mechanisms by which NRG1 and AKT1 alone or in combination contribute to susceptibility for schizophrenia remain unclear. The objective of this study is to characterize behavioral and cognitive phenotypes of AKT1-NRG1 single and double mutant mice through a comprehensive battery of behavioral tasks. NRG1 heterozygous mice, AKT1 heterozygous mice, NRG1-AKT1 double heterozygous mice, and wild type mice were generated by NRG1 heterozygous and AKT1 heterozygous breeding pairs. On postnatal day 67~77, a battery of behavioral assays was conducted. Our preliminary data indicate that male AKT1-NRG1 single and double mutant mice displayed normal behavioral profiling of locomotion, anxiety-like behavior, sensorimotor gating function, and episodic-like memory compared with their wild-type controls. In contrast, double mutant mice did not display social preference compared with the other 3 groups. Further experiments are in progress. These mutant mice provide a simple and relatively straightforward approach to investigate the effects of gene-gene interaction in the pathogenesis of schizophrenia and schizophrenia-like behaviors.