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Poster Sessions

学習と記憶I
Learning and Memory I

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開催日
2010年09月02日(木)
時 間
11:00 - 12:00
会 場
Poster Room 2

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ショウジョウバエのchico遺伝子の変異はキノコ体の神経の可塑性を阻害する。
Mutation in chico, a Drosophila insulin receptor substrate homolog, disrupts neural plasticity of the mushroom bodies.

演題番号 : P1-m27

長野 慎太郎 / Shintaro Nagano:1,2 上野 耕平 / Kohei Ueno:1 齊藤 実 / Minoru Saitoe:1 

1:東京都神経科学総合研究所 / Tokyo metropolitan institute for neuroscience, Tokyo, Japan 2:首都大学東京大学院 理工学研究科 生命科学専攻 / Tokyo Metropolitan University, Department of Biological science. Tokyo, Japan 

 

In Drosophila olfactory aversive conditioning, flies learn to associate an odor with noxious electrical shock, and this association is formed in the mushroom bodies (MBs). While odor information is delivered to the MBs via projection neurons forming the antennal lobes (ALs), shock information is delivered to the MBs via ascending ventral nerve cord (VNC). And thereby odor evoked neural activity in the MBs are enhanced after olfactory conditioning.We have recently found that mutation in chico gene, which encodes a Drosophila homolog of insulin receptor substrate, results in disrupted olfactory memory formation.We identified that chico gene expression in the MBs were required for olfactory memory formation. To address the effects of chico mutation on neuronal plasticity of the MBs, we developed the imaging system for dissociated Droophila brain, which were expressed G-CaMP, a fluorescence Ca2+ indicator protein, in the MBs. In our imaging system, Ca2+ responses in the α lobes of the MBs are facilitated upon simultaneous stimulation of ALs and VNC in both wild-type and chico mutants. However, while facilitated ALs-induced Ca2+ responses after simultaneous stimulation were persisted in wild-type, it was not in chico mutants. These results suggest that chico is required for retention of associated information at the MBs

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