演題番号 : P1-m21
富澤 はるな / Haruna Tomizawa:1 栗田 真季 / Maki Kurita:1 松澤 大輔 / Daisuke Matsuzawa:1 松田 真悟 / Shingo Matsuda:1 石井 大典 / Daisuke Ishii:1 清水 栄司 / Eiji Shimizu:1
1:千葉大院・医・認知行動生理 / Department of Cognitive Behavioral Physiology, Chiba University Graduate School of Medicine
Recent researches have revealed that the amygdala plays a central role in the acquisition and the expression of fear, although few studies have investigated comprehensive gene expression along with the acquisition of fear memory. To investigate gene expression following fear conditioning, we examined cued fear conditioning paradigm in C57BL/6J male mice. Two hours after fear conditioning, the amygdala was dissected out bilaterally from the coronal slices. Then total RNA was purified. Gene expression was analysed by Serial Analysis of Gene Expression (SAGE) resulting 707 up-regulated genes and 1858 down-regulated genes in the fear conditioned sample relative to the control. We found that cytoskeleton related genes (Actb, MAP1), and several vesicle exocytosis related genes (Stx12, Cplx2, and SLC17A6) were up-regulated, and neurodegenerative disease related genes (Gsk3b, Hap1) were down-regulated in the amygdala after fear conditioning. Changes in cytoskeletal and exocytotic gene expressions might be involved in the fear memory acquisition.