演題

Novel cytological diagnosis of peritoneal wash for gastric cancer by fluorescence-emitting virus TelomeScan

[演者] 香川 俊輔:1
[著者] 渡邉 めぐみ:1, 石田 道拡:1, 黒田 新士:1, 菊地 覚次:1, 田澤 大:1, 岸本 浩行:1, 桒田 和也:1, 久保田 哲史:1, 掘 直人:1, 田邊 俊介:1, 西﨑 正彦:1, 浦田 泰生:2, 藤原 俊義:1
1:岡山大学消化器外科, 2:オンコリスバイオファーマ株式会社

The uneven shape of the peritoneal cavity makes it impossible for the entire cavity to be thoroughly inspected and difficult for the surgeons to definitively judge whether the peritoneal cavity is completely free of metastatic foci. Consequently, peritoneal lavage cytology is needed for the indirect diagnosis or prediction of peritoneal metastasis. However, there is still room for improvement in the diagnosis of peritoneal lavage cytology. TelomeScan which is an adenovirus engineered to replicate and express GFP only in telomerase-activating malignant cells, can detect viable cancer cells even among numerous normal cells. We examined whether TelomeScan is applicable to detecting free gastric cancer cells in peritoneal wash using clinical samples. Peritoneal washes obtained from 62 gastric cancer patients were infected with TelomeScan for 24 hours, and number of GFP-positive cells was determined. The methods were established using the initial 16 samples. The remaining 46 cases were analyzed with clinical data. In these cases, one or more GFP-positive cells could be detected in 21 cases. The cases detected GFP-positive cells showed a significant worse prognosis when compared to the negative cases, especially in the cytology positive cases. In conclusion, we were able to detect gastric cancer cells as GFP-positive cells in peritoneal wash using TelomeScan. The presence of GFP-positive cells in peritoneal wash was associated with worse prognosis. The TelomeScan-guided cytological test may have important clinical implication as prognostic biomarkers in gastric cancer.
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