演題詳細

ポスター / Poster

ポスター 49 (Poster 49) :ウイルス感染症

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日程
2013年10月11日(金)
時間
16:50 - 17:50
会場
ポスター会場 / Poster (ロイトン札幌 3F エメラルドABCD)
座長・司会
古川 雄祐 (Yusuke Furukawa):1
1:自治医科大学分子病態治療研究センター 幹細胞制御研究部
 
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Analysis of viral infection by multiplex PCR in immunosuppressed patients with liver dysfuncton

演題番号 : PS-1-371

伊藤 仁也 (Kiminari Ito):1、常峰 紘子 (Hiroko Tsunemine):1、赤坂 浩司 (Hiroshi Akasaka):1、坂根 恵美子 (Emiko Sakane):1、小高 泰一 (Taiichi Kodaka):1、清水 則夫 (Norio Shimizu):2、高橋 隆幸 (Takayuki Takahashi):1

1:Dept.Hematology, Shinko Hospital,Kobe,Japan、2:Dept of Virology,Medical Research Institute,Tokyo Medical and Dental Univ,Japan

 

Objective:The patients in an immunosuppressive state have often unexplained liver dysfunction .The identification of viruses that cause liver dysfunction is difficult because methods that identify these viruses simultaneously have not been established. The aim of this study was to quickly and simultaneously identify multiple virus species.Patients and Methods:A total of 82 patients with unexplained liver dysfunction and undetermined inflammation were examined. The majority of patients had hematologic malignancies, and some had undergone bone marrow transplantation. First, qualitative polymerase chain reactions (PCR) were performed to detect 12 species of DNA virus in whole blood. When a specific PCR signal was obtained, quantitative real-time PCR was performed. In addition, 6 RNA hepatitis viruses were directly assayed by real-time PCR. These 2 PCR steps were completed within 1 h. Results:In 82 patients with liver dysfunction, the most frequently detected virus was transfusion transmitted virus (92.7%), which was followed by CMV (30.5%),HHV-6(29.2%), EBV(10.9%), HBV(7.3%),and rarely HCV and HGV and HHV-7 (2.4%). Mostly patients with liver dysfunction caused by herpes viruses had mild liver dysfunction, similar viremia was observed. But the patients reactivated with hepatitis virus B and C had severe liver dysfunction.Conclusion:Multiplex viral PCR assay was a useful tool for quickly identifying viruses that possibly cause liver dysfunction. It was also important that liver dysfunction acted as a proband that led to the discovery of serious viremia.

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