演題詳細

ポスター / Poster

ポスター 49 (Poster 49) :ウイルス感染症

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日程
2013年10月11日(金)
時間
16:50 - 17:50
会場
ポスター会場 / Poster (ロイトン札幌 3F エメラルドABCD)
座長・司会
古川 雄祐 (Yusuke Furukawa):1
1:自治医科大学分子病態治療研究センター 幹細胞制御研究部
 
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Early diagnosis of viral infection by multiplex PCR assays in allogeneic stem cell transplantation

演題番号 : PS-1-370

神前 愛子 (Aiko Kozaki):1、長谷川 大一郎 (Daiichiro Hasegawa):1、宮田 憲二 (Kenji Miyata):1、越智 聡史 (Satoshi Ochi):1、斉藤 敦郎 (Atsuro Saito):1、石田 敏章 (Toshiaki Ishida):1、矢内 友子 (Tomoko Yanai):1、川崎 圭一郎 (Keiichiro Kawasaki):1、伊藤 仁也 (Kiminari Ito):2、清水 則夫 (Norio Shimizu):3、小阪 嘉之 (Yoshiyuki Kosaka):1

1:Department of Hematology and Oncology, Kobe Children's Hospital,Japan、2:Department of Hematology, Shinko Hospital, Kobe, Japan、3:Department of Virology, Medical Research Institute, Tokyo Medical and Dental University

 

Viral infections are important causes of morbidity and mortality after allogeneic hematopoietic stem cell transplantation(HSCT). The aim of this study is to evaluate the efficacy of early diagnosis of viral infection in allogeneic HSCT recipients. Between May 2011 and March 2013, 21 children with a median age of 5.7 years were studied in our hospital. The allografts included 4 bone marrows (BM) from HLA matched siblings and 17 alternatives (13 unrelated BM, 2 cord blood, 2 mismatched related BM/peripheral blood, respectively). The conditioning regimens were myeloablative in 6 patients and non-myeloablative in 15. After HSCT, qualitative PCR were weekly performed to detect 12 species of DNA virus in whole blood, and in urine if patients have cystitis. Quantitative real-time PCR was also performed when a specific virus was amplified. Ten CMV, 9 HHV-6, 5 BKV, 2 JCV, 1 EBV and 2 ADV were detected respectively. Among them, 3 patients developed BKV-associated cystitis, while a patient was complicated with CMV pneumonia. In a patient who developed CMV pneumonia, before positive antigenemia, quantitative PCR was first positive in whole blood. None of 21 (0%) patients developed HSCT-related death. Quantitative real-time PCR could detect viral reactivation/infections prior to disease development. Comprehensive virus monitoring after HSCT could be a useful diagnostic tool for children in whom histological diagnosis might be less feasible. Our pilot study showed that early diagnosis of viral reactivation/infections might contribute to reduction of HSCT-related death in HSCT recipients.

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