演題詳細

一般口演 / Oral Session

一般口演 107 (Oral Session 107) :ウイルス感染症/血球貪食症候群

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日程
2013年10月13日(日)
時間
15:00 - 16:00
会場
第14会場 / Room No.14 (札幌市教育文化会館 3F 研修室305)
座長・司会
森 慎一郎 (Shinichiro Mori):1
1:聖路加国際病院 血液腫瘍内科
 
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New class small RNAs associated with human Ago1: Its sorting mechanism, function, processing

演題番号 : OS-3-181

高倉 光平 (Kohei Takakura):1,2、奥山 一生 (Kazuki Okuyama):1、高松 昌子 (Masako Takamatsu):1、笠原 麻衣 (Mai Kasahara):1、平林 直己 (Naomi Hirabayashi):1、金井 昭教 (Akinori Kanai):3、松井 啓隆 (Hirotaka Matsui):3、稲葉 俊哉 (Toshiya Inaba):3、幸谷 愛 (Ai Koutani):1

1:Department of Regenerative Medicine, Hematological Malignancy, Tokai University、2:Department of Biosciences, School of Science, Kitasato University、3:Department of Molecular Oncology and Leukemia Program Project, Hiroshima University

 

Argonaute proteins (Ago1-4) are essential components of RNA-induced silencing complex (RISC). In lower organisms, the siRNA and miRNA pathways diverge due to sorting mechanisms that direct distinct small RNA duplexes into specific Ago-RISCs. However, such sorting mechanisms appear to be lost in mammals. In order to investigate whether the sorting system is affected by viral infection, we compared the content of deep sequenced RNA extracted from Ago1- and Ago2-immunoprecipitated(i.p.) cell lysate of EBV infected cells. Consistent with previous observations, sequence tags derived from the miRNA loci both in EBV and human, globally associate in approximately equivalent amounts with Ago1 and Ago2. Interestingly, some unique sequence tags derived from tandem loci in the human genome associate with Ago1 but not or few if any Ago2. The majority of them have not been registered as miRNAs. The results was confirmed by real time PCR analysis, which displayed that the amount of the small RNAs(sRNAs) was significantly high in Ago1-i.p. than Ago2-i.p.sample When Ago1 was knocked down, the expression of the Ago1-specific sRNAs dramatically decreased. Taken these together, even in mammals, unknown sorting mechanism in Ago1-4 family still works at least in viral infected cells. Moreover, Ago1-specific sRNAs imply vital roles in some aspects of mammalian biology. The mechanistic analysis of the processing, stability, and function of these Ago1 specific sRNAs in EBV infected cells are now investigated.

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