演題詳細

一般口演 / Oral Session

一般口演 105 (Oral Session 105) :免疫制御

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日程
2013年10月13日(日)
時間
15:00 - 16:00
会場
第13会場 / Room No.13 (札幌市教育文化会館 3F 研修室301)
座長・司会
渋谷 彰 (Akira Shibuya):1
1:筑波大学医学医療系 免疫学
 
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TLR2-dependent induction of IL-10 and Foxp3Tregs prevents Pam2 lipopeptide anti-tumor immunity

演題番号 : OS-3-174

岡田 耕平 (Kohei Okada):1、山崎 小百合 (Sayuri Yamazaki):2、丸山 晃 (Akira Maruyama):3、松本 美佐子 (Misako Matsumoto):3、八木田 秀雄 (Hideo Yagita):4、豊嶋 崇徳 (Takanori Teshima):1、瀬谷 司 (Tsukasa Seya):3

1:Department of Hematology, Hokkaido University Graduate School of Medicine、2:Department of Geriatric and Environmental Dermatology, Nagoya City University, Nagoya, Japan、3:Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan、4:Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan

 

Pam2 lipopeptides, 16 S-[2,3-bis(palmitoyl)propyl]cysteine (Pam2Cys) lipopeptides, act as TLR2/TLR6 ligands and activate natural killer cells (NKs) and dendritic cells (DCs) to produce inflammatory cytokines and cytotoxic NK activity in vitro. However, we here found that systemic injection of Pam2 lipopeptides was not effective for the suppression of NK-sensitive B16 melanoma in vivo. When we investigated the immune suppressive mechanisms, systemic injection of Pam2 lipopeptides induced IL-10 in a TLR2-dependent manner. The Pam2 lipopeptides increased the frequencies of Foxp3+CD4+ regulatory T cells (T reg) in TLR2- and IL-10- dependent manner. The T reg from Pam2-lipopeptide injected mice maintained suppressor activity. Pam2 lipopeptides plus depletion of T reg with anti-CD25 mAb improved tumor growth compared to Pam2 lipopeptides alone. In conclusion, our data suggest that systemic treatment of Pam2 lipopeptides promotes IL-10 production and T reg function, which suppresses the effective induction of anti-tumor immunity in vivo. It is necessary to develop an adjuvant that does not promote IL-10 and T reg function in vivo for future establishment of anti-cancer vaccine.

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