演題詳細

一般口演 / Oral Session

【E】一般口演 21 (Oral Session 21) :Hematopoietic Cell Transplantation

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日程
2013年10月11日(金)
時間
14:25 - 15:25
会場
第5会場 / Room No.5 (さっぽろ芸文館 3F 清流)
座長・司会
石山 謙 (Ken Ishiyama):1
1:Div. Hematology, Tokyo Metropolitan Ohtsuka Hospital, Tokyo, Japan
 
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Successful isolation of hematopoietic stem cells from cryopreserved placental tissue

演題番号 : OS-1-106

池田 裕一 (Hirokazu Ikeda):1、小金沢 征也 (Masaya Koganezawa):1、松野 良介 (Ryosuke Matsuno):1、外山 大輔 (Daisuke Toyama):1、磯山 恵一 (Keiichi Isoyama):1

1:Department of Pediatrics, Showa University Fujigaoka Hospital

 

Introduction: We have previously reported a significant amount of viable HSCs can be recovered from fresh human term placenta avoiding maternal cell contamination using a closed perfusion system. In this study, we demonstrated a novel isolation techinque to collect HSCs from cryopreserved placental tissue.
Material and Methods: A total of 26 normal placentas were obtained from consenting mothers undergoing cesarean delivery of full-term healthy infants. After delivery, the placentas were quickly cleaned with sterile saline solution, covered with sterile gauze, and cryopreserved in a double Ziploc bag at -80C° for 30 days. Following storage, the placentas were thawed and then perfused with 500 ml heparinized medium for 60 min. The HSCs were recovered from the perfusate by centrifugation and immediately thereafter the effects of cryopreservation were evaluated.
Results: Using this perfusion system, we obtained an average of 4.9± 9.7×107 nucleated cells and a total of live CD34+/CD45 cells of 0.54± 0.22×106 from cryopreserved-thawed placental tissue. Compared with prefreeze, cryopreservation did not significantly decrease 7-aminoactinomycin D viability, the proportion of CD34+ cells, or the CD34+/CD38- cells, which is an indicator of primitive HSCs. In addition, bacterial and fungal contamination was not observed.
Conclusion: Our group successfully isolated HSCs from 30-day-old cryopreserved placental tissue using a closed-circuit placental perfusion system. We believe that this novel technique will facilitate a new source of HSCs.

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