演題詳細

ポスター / Poster

【E】ポスター 43 (Poster 43) :GVHD/Chimerism

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日程
2013年10月11日(金)
時間
16:50 - 17:50
会場
ポスター会場 / Poster (ロイトン札幌 3F エメラルドABCD)
座長・司会
岡本 康裕 (Yasuhiro Okamoto):1
1:Department of Pediatrics, Kagoshima University Hospital, Japan
 
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Protective role of host mast cells against acute graft-versus-host disease

演題番号 : PS-1-324

荒木 来太 (Raita Araki):1、西村 良成 (Ryosei Nishimura):1、前馬 秀昭 (Hideaki Maeba):1、黒田 梨絵 (Rie Kuroda):1、藤木 俊寛 (Toshihiro Fujiki):1、馬瀬 新太郎 (Shintaro Mase):1、小泉 晶一 (Shoichi Koizumi):1、谷内江 昭宏 (Akihiro Yachie):1

1:Department of Pediatrics, Kanazawa University, Kanazawa, Japan

 

Originally mast cells have been known as effector cells in the IgE-mediated allergic responses. In addition, recent studies demonstrated that mast cells exerted pro-inflammatory or anti-inflammatory effects in complicated immune response depending on the situation. In allogeneic hematopoietic stem cell transplantation (HSCT), exact role of mast cells in graft-versus-host disease (GVHD) remains unclear. First we investigated whether host mast cells protect acute GVHD or not in a murine HSCT model using mast cell-deficient mice. When lethally irradiated wild type (WT) B6 or mast cell deficient mice were transplanted with bone marrow cells and spleen cells from Balb/c mice, mast cell-deficient B6 host mice showed significantly lower survival rate than WT B6 mice (p<0.05) due to acute GVHD. Next, bone marrow derived cultured mast cells (BMCMCs) inhibited mixed lymphocyte reaction (MLR) in a dose-dependent manner, when added to the co-culture (Stimulator: DCs from B6, Responder: spleen cells of Balb/c, BMCMCs from B6). Although mast cells contain many kinds of substances to regulate immune reactions, we have clearly shown that BMCMCs dominantly regulated the alloreaction in a cell contact-dependent manner by using transwell system. In conclusion, mast cells in recipient mice could have protective role against acute GVHD due, in part, to cell-to-cell contact inhibition of alloreaction. The understanding of mechanisms by which mast cells suppress alloreaction might lead us to new therapeutic strategy for GVHD.

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