演題詳細

ポスター / Poster

ポスター 38 (Poster 38) :血栓症・その他 (Thrombosis and Others)

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日程
2013年10月11日(金)
時間
16:50 - 17:50
会場
ポスター会場 / Poster (ロイトン札幌 3F ロイトンホールABCD)
座長・司会
小嶋 哲人 (Tetsuhito Kojima):1
1:名古屋大学大学院医学系研究科
 
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Electromagnetic irradiation to PRP by Pipetector preserves platelet's viability

演題番号 : PS-1-287

山本 正雅 (Naomasa Yamamoto):1、箭内 美穂子 (Mihoko Yanai):1、遠藤 将 (Syo Endo):1、江澤 友子 (Yuko Ezawa):1、中楯 奨 (Shou Nakadate):2、熊野 活行 (Katsuyuki Kumano):3、米原 典史 (Norifumi Yonehara):4、小池 勇一 (Yuichi Koike):5

1:Dept. of Biochemistry, School of Pharmaceutical Sciences, Ohu University, Japan、2:Dept. of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Ohu University, Japan、3:Japan Syatem Planing Co., Ltd. Tokyo, Japan、4:Dept. of Pharmacology, School of Dentistry, Ohu University, Japan、5:Dept. of Drug Metabolism and Clinical Pharmacokinetics, School of Pharmaceutical Sciences, Ohu University, Japan

 

Objective:Pipetector radiates electromagnetic wave and it can induce the reduction of Fe(III) ions in drinking water, which physical effects concomitantly forms precipitation of ferrous sludge by forming negatively charged water. We succeeded to apply this technology to reduce oxidative stress in blood in vivo, and it effectively reduced the oxidative stress (d-ROM) in blood. In this study, we studied the effect of Pipetector's radiation on platelet functions in vitro. Methods:Pipetectors (PT-20DS/PT-30DS) were obtained from Japan System Planning. Radiation of Pipetector was evaluated optically by increase of absorbance at 210 nm of water. PRP was irradiated for 0-24 hours at room temperature, and then platelet aggregations were measured by Hematracer. Oxidative stress in circulating blood (d-ROM) was measured by Fras4 (Wismerll). NOx-release from platelets was measured by ENO-200 (Eicom) expressed as NO2 and NO3.Results:Irradiation to water by Pipetector increased absorbance at 210 nm, which was associated with negatively charged water formation. Irradiation to PRP induced the platelet's viability (p<0.3%) as judged by platelet aggregability induced by collagen, shape change and NOx-release. NOx was released from aggregating platelets induced by collagen and reached maximal at 5 min after stimulation. NOx-release was also preserved after treatment with irradiation.Conclusion:Irradiation to PRP in vitro can prolong to preserve platelet's viability, suggesting that preservation of platelet's viability by Pipetector may reflect to the reduction of oxidative stress in vivo.

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