演題詳細

ポスター / Poster

ポスター 38 (Poster 38) :血栓症・その他 (Thrombosis and Others)

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日程
2013年10月11日(金)
時間
16:50 - 17:50
会場
ポスター会場 / Poster (ロイトン札幌 3F ロイトンホールABCD)
座長・司会
小嶋 哲人 (Tetsuhito Kojima):1
1:名古屋大学大学院医学系研究科
 
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Host protein C inhibitor inhibits tumor cell growth, but promotes tumor cell metastasis

演題番号 : PS-1-285

秋田 展幸 (Nobuyuki Akita):1、岡本 貴行 (Takayuki Okamoto):2、西岡 淳二 (Junji Nishioka):3、鈴木 宏治 (Koji Suzuki):4、林 辰弥 (Tatsuya Hayashi):5

1:Department of Clinical Engineering, Suzuka University of Medical Science, Japan、2:Department of Molecular Pathobiology, Mie University Graduate School of Medicine, Japan、3:Department of Clinical Laboratory, Suzuka University of Medical Science, Japan、4:Faculty Pharmaceutical Science, Suzuka University of Medical Science, Japan、5:Department of Biochemistry, Mie Prefectural College of Nursing, Japan

 

Protein C inhibitor (PCI), a member of the SERPIN family, is expressed in various human tissues, including liver and kidneys. PCI mainly functions as a procoagulant by inhibiting activated protein C (APC). We have shown that PCI expressed by tumor cells inhibits tumor cell growth and metastasis in mice, in which expression of PCI is restricted to the reproductive organs. In the present study, to clarify the influence of host PCI on tumor cell growth and metastasis we observe the growth and metastasis of B16 melanoma (B16) cells and PCI-expressing B16 cells in wild type and in human PCI-transgenic (hPCI-TG) mice, which show similar tissue distribution of PCI to humans. Growth of intracutaneously-injected B16 cells was significantly faster in wild-type mice than in hPCI-TG mice, and PCI expression in B16 cells decreased their growth rate in both types. Surprisingly, metastatic behavior of intravenously-injected B16 cells was higher in hPCI-TG mice than in wild type mice, and PCI expression in B16 cells decreased their metastatic potential in both types. Furthermore, metastatic behavior observed in hPCI-TG mice was reduced by treatment with anti-human PCI IgG, soluble thrombomodulin or APC for 3 consecutive days including the day B16 cells were injected. These findings suggest that PCI in hPCI-TG mice inhibits tumor growth, but conversely promotes tumor metastasis by its procoagulant property, and that PCI expression by tumor cells decreases their growth and metastatic potentials not only in wild mice, but also in hPCI-TG mice.

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