演題詳細

一般口演 / Oral Session

一般口演 32 (Oral Session 32) :巨核球造血・血小板減少症

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日程
2013年10月11日(金)
時間
15:25 - 16:25
会場
第13会場 / Room No.13 (札幌市教育文化会館 3F 研修室301)
座長・司会
冨山 佳昭 (Yoshiaki Tomiyama):1
1:大阪大学医学部附属病院 輸血部
 
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Bone marrow megakaryocytes generate two types of intra-vascular protrusion

演題番号 : OS-1-162

古和田 周吾 (Shugo Kowata):1、磯貝 純夫 (Sumio Isogai):2、村井 一範 (Kazunori Murai):1、伊藤 薫樹 (Shigeki Ito):1、遠山 稿次郎 (Koujirou Tohyama):3、人見 次郎 (Jiro Hitomi):2、石田 陽治 (Yoji Ishida):1

1:Hematology/Oncology, Iwate Medical University, Japan、2:Anatomy and Human Embryology, Iwate Medical University、3:Center for Electron Microscopy and Bio-Imaging Reserch, Iwate Medical University

 

Background: Although proplatelet model is a unique model to explain how MKs produce the platelets, the large fragments without characteristics of proplatelet have been found in BM sinusoid in recent intra-vital imaging studies. There remains still unclear whether the large fragments are produced from proplatelets. Materials and methods: To observe the detailed process of intra-vascular protrusions from BM MK in living mice, we visualised the dynamic process in intact bone marrow of EGFP transgenic mice using a two-photon intra-vital microscope(TPIVM). We also analysed BM using conventional methods. Resuts: MKs extended a long and thin protrusion with several-beaded appearance into the vessel lumen, consistent with characteristics of typical proplatelets previously reported. We further documented thick protrusions with large volume, dissimilar from the proplatelet in the point of forming dynamics by analysing sequential images from TPIVM. In electron microscopic study, the thick protrusions were characterised by abundant demarcation membrane system and organelles inside the protrusion, and by a peripheral zone surrounding the entire area of the protrusions. In addition, mice under thrombocytopenia showed that BM MKs produce predominantly the thick protrusions rather than proplatelets. Conclusion: Our results suggest that BM MKs have two releasing processes of platelet production via intra-vascular protrusions, and generate intravascular thick protrusion and its progeny in the response to platelet demand.

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