演題詳細

一般口演 / Oral Session

一般口演 32 (Oral Session 32) :巨核球造血・血小板減少症

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日程
2013年10月11日(金)
時間
15:25 - 16:25
会場
第13会場 / Room No.13 (札幌市教育文化会館 3F 研修室301)
座長・司会
冨山 佳昭 (Yoshiaki Tomiyama):1
1:大阪大学医学部附属病院 輸血部
 
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Comparison between subcutaneous preadipocyte- and bone marrow megakaryocyte-derived platelets

演題番号 : OS-1-161

田中 達也 (Tatsuya Tanaka):1、小野-宇留賀 友佳子 (Yukako Ono-Uruga):2、猪狩 敦子 (Atsuko Igari):3、森木 隆典 (Takanori Moriki):3、池田 康夫 (Yasuo Ikeda):1、須田 年生 (Toshio Suda):4、合田 亘人 (Nobuhito Goda):1、村田 満 (Mitsuru Murata):3、松原 由美子 (Yumiko Matsubara):4

1:Life Science and Medical BioScience, Waseda University, Tokyo, Japan、2:Kanagawa Academy of Science and Technology, Kawasaki, Japan、3:Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan、4:Department Cell Differentiation, Keio University School of Medicine, Tokyo, Japan

 

We previously reported the platelet generation derived from preadipocyte cell lines, 3T3-L1 and OP9, and preadipocytes obtained from human and mouse adipose tissues. In this study, we compared the number and function of platelets derived from subcutaneous preadipocytes and bone marrow (BM) megakaryocytes (MKs). The preadipocytes and BM mononuclear cells were obtained from individual mouse and were cultured in MK lineage induction media for their differentiation into platelets. The total number of platelets obtained from a mouse, assessed by CD41+ platelet-sized cells, was 22.6±3.2 x 105 for preadipocyte-derived cells and 3.9±1.0 x 105 for BM MK-derived cells (n=3, p=0.0045). To examine the ability for platelet release in vivo, CFSE-labeled MKs were infused into irradiated thrombocytopenic mice. The ability, assessed by the number of CFSE+/CD41+ platelet-sized cells in a blood sample after 90 min of infusion, was similar between preadipocyte- and BM MK-derived cells (n=3, p=0.2850). To analyze the platelet function, blood sample from infused mouse was perfused on a collagen-coated chip under flow condition using the Total Thrombus-formation Analysis System. The frequency of incorporated cells in the infused cells, assessed by CFSE+/CD41+ platelet-sized cells, was higher in preadipocyte-derived cells (77.8±5.1%) than in BM MK-derived cells (52.2±5.3%; n=3, p=0.0257). In summary, we obtained a greater number of platelets derived from preadipocytes than BM MKs in an individual mouse. The preadipocyte-derived platelets might have higher function than BM MK-derived platelets.

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