演題詳細

一般口演 / Oral Session

一般口演 32 (Oral Session 32) :巨核球造血・血小板減少症

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日程
2013年10月11日(金)
時間
15:25 - 16:25
会場
第13会場 / Room No.13 (札幌市教育文化会館 3F 研修室301)
座長・司会
冨山 佳昭 (Yoshiaki Tomiyama):1
1:大阪大学医学部附属病院 輸血部
 
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Composition of cell surface glycans of thrombocytes in a cold-induced thrombocytopenic frog model

演題番号 : OS-1-160

柏瀬 奈央 (Nao Kashiwase):1、谷崎 祐太 (Yuta Tanizaki):1,2、前川 峻 (Shun Maekawa):3、加藤 尚志 (Takashi Kato):1,3

1:Integrative Bioscience and Biomedical Engineering, Waseda University, Tokyo, Japan、2:Research Fellowships of Japan Society for the Promotion of Science、3:Department of Biology, School of Education, Waseda University, Tokyo, Japan

 

Rapid reduction in platelet viability during cold-storage has been the major conern in platelet transfusions (Murphy et al. Blood, 1970). To investigate the clearance mechanism of peripheral platelets in low-temperature bodies, we attempted to establish hypothermic animal models by using ectothermic animals. We then reported a cold-induced pancytopenia at 5°C in African clawed frogs, Xenopus laevis. (Maekawa et al. J. Exp. Biol., 2012). Since Hoffmeister et al. (J. Thrombosis and Haemostasis, 2011) reported that chilled blood platelets rapidly left from the circulation by changing the structures of N-linked glycans on platelets, we hypothesized that the structural change of N-linked glycans on the surface of thrombocytes permits this clearance. The frogs at 5°C decreased peripheral thrombocytes to eighth part of the initial counts in 4 hours. It was more rapid than we expected, therefore we examined whether they moved over to specific organs. Immunostaining using thrombocyte specific monoclonal antibody (T12) revealed that nine-fold increase in the number of thrombocytes was detected in the spleen after 4 hours of 5°C exposure. We then analyzed the change of structures of N-linked glycan residues. Flowcytometric lectin stainings of thrombocytes in the circulation and the spleen demonstrated that thrombocytes moved to the spleen from the circulation by changing their N-linked glycan structures. Likewise, the possibility of the shortened life span of peripheral thrombocytes under cold-temperature, which caused hypothermia, will be discussed.

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