演題詳細

一般口演 / Oral Session

【E】一般口演 13 (Oral Session 13) :Malignant Lymphoma:Genomic Alteration

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日程
2013年10月11日(金)
時間
09:30 - 10:30
会場
第9会場 / Room No.9 (ロイトン札幌 1F キャッスル)
座長・司会
渡邊 俊樹 (Toshiki Watanabe):1
1:Laboratory of Tumor Cell Biology, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Japan
 
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The regulation of ''inflammatory niche'' with tumor derived small RNAs

演題番号 : OS-1-65

山川 奈津子 (Natsuko Yamakawa):1、横山 和明 (Kazuaki Yokoyama):2、Jun Lu:3、今留 謙一 (Ken-Ichi Imadome):4、渡辺 俊樹 (Toshiki Watanabe):5、堀江 良一 (Ryouichi Horie):6、穂積 勝人 (Katsuto Hozumi):2、八幡 崇 (Takashi Yahata):1、安藤 潔 (Kiyoshi Ando):3、中村 直哉 (Naoya Nakamura):7、幸谷 愛 (Ai Koutani):1

1:Dept. of Regenerative Med., Tokai Univ. Sch. of Med., Japan、2:Dept. of Imm., Tokai Univ. Sch. of Med.、3:Dept. of Hematology, Tokai Univ. Sch. of Med.、4:Nat. Res. Inst. for Child Health and Dev.、5:Lab. of Tumor Cell Biol., The Univ. of Tokyo、6:Dept. of Hematology, Kitasato Univ.、7:Dept. of Pathology, Tokai Univ. Sch. of Med.

 

Introduction: EB virus (EBV) is associated with heterogeneous lymphomas. Hodgkin's lymphoma (HL) cells are embedded in non-neoplastic bystanders: B and T cells, macrophages. Without these bystander cells, the lymphoma cells are incapable of being engrafted in immunodeficient mice. In this context, the bystanders are tumor-supportive ''inflammatory niche''. Recently, EBV-infected cells produce exosomes that contain EBV specifically encoded miRNAs (EBV-miRNAs). The miRNAs are transferred to cells, and involved in tumor metastasis. However, the detailed mechanism is unknown. Accordingly, we hypothesized that exosomal EBV-miRNAs might redirect tumor surrounding immune cells from tumor reactive into tumor-supportive ''inflammatory niche''. Methods: We evaluated the expression of EBV-miRNAs in EBV+HL clinical specimens by in situ hybridization, their functional characterization in vitro, and their effects on persistent infection and tumor development in vivo. Results and discussion: The exosomes produced by EBV+ cells (EBV-Ex) were harvested either from the type III (EBV-miRNA rich) or type I (EBV-miRNA vacant) EBV-infected cells. The EBV-miRNAs effects were potent on monocyte/macrophage (Mo/Mf) in inducing CD69, IL-10, and TNF, suggesting that EBV-miRNAs might polarize Mo/Mf into tumor associated Mf (TAM). EBV-miRNAs suppress cell proliferation in vitro, while, they are required to develop LPD. Most importantly, exosomal EBV-miRNAs were transferred in Mf in human EBV+ HL samples. Now we are searching for the target of EBV-miRNAs and the effect on ''inflammatory niche''.

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